Within
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Reason

"Chance is the very guide of life"

"In practical medicine the facts are far too few for them to enter into the calculus of probabilities... in applied medicine we are always concerned with the individual" -- S. D. Poisson

May 15, 2003

Chemical Genetic Modifier Screens: Small Molecule Trichostatin Suppressors as Probes of Intracellular Histone and Tubulin Acetylation



Histone deacetylase (HDAC) inhibitors are being developed as new clinical agents in cancer therapy, in part because they interrupt cell cycle progression in transformed cell lines. To examine cell cycle arrest induced by HDAC inhibitor trichostatin A (TSA), a cytoblot cell-based screen was used to identify small molecule suppressors of this process. TSA suppressors (ITSAs) counteract TSA-induced cell cycle arrest, histone acetylation, and transcriptional activation. Hydroxamic acid-based HDAC inhibitors like TSA and suberoylanilide hydroxamic acid (SAHA) promote acetylation of cytoplasmic alpha-tubulin as well as histones, a modification also suppressed by ITSAs. Although tubulin acetylation appears irrelevant to cell cycle progression and transcription, it may play a role in other cellular processes. Small molecule suppressors such as the ITSAs, available from chemical genetic suppressor screens, may prove to be valuable probes of many biological processes.

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1 comment:

rmudgett said...

Napa, CA (PRWEB) February 3, 2006 -- California Peptide Research, Inc. (www.californiapeptide.com), a peptide synthesis company located in Napa, California, announced today that they have synthetic obestatin available for bio medical research. Obestatin is a new discovery in the search of a real treatment for obesity, and shows great promise.

The beautiful Napa Valley, a place that most folks associate with fine wines, beautiful scenery and bed and breakfasts is also a place that produces peptides for research. Among the many peptides produced there, is one that is a recent discovery and may help millions that want to lose weight.

The peptide is called obestatin and it was discovered by researchers at the Stanford University School of Medicine. It is one of many appetite-regulating hormones found in several mammals, including humans and may be the key to an actual treatment for obesity.

Obestatin is a sibling hormone to the hunger hormone known as ghrelin, which is an appetite-booster made in the stomach. However, scientists are calling obestatin the "anti-ghrelin" because it works exactly the opposite of ghrelin.

Early tests of the effects of obestatin in rats shows great promise. Rats injected with obestatin ate less and lost weight. More research needs to be done, of course in the months and years ahead to ensure it does in humans what it apparently is doing for the lab rats.

More information for researchers can be found on California Peptide Research, Inc.'s website www.californiapeptide.com.

California Peptide Research, Inc. offers a wide variety of peptides including Obestatin, Adrenomedullins, Endothelins, Endothelin Antagonists, Enzyme Substrates and Inhibitors, Sulfated Peptides such as CCK and Caerulien, and a great number of Beta Amyloids which have been found to offer useful tools in Alzheimer's research.